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Prolotherapy is a procedure that involves injecting proliferate solution to treat connective tissue injuries and cause a growth of the collagen issue. The goal is to strengthen ligaments damaged by trauma or injury and to gradually reduce pain.

The proliferate solution injected into the tissue causes a cascade of biological reactions. The release of pre-collagen growth factor (PGF) encourages growth of fibroblasts into the injection area which then secrete the collagen tissue. The process allows natural healing to take place which can take between 6 to 8 weeks to complete.